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分别将Ca(Ⅱ)和Zn(Ⅱ)加入到壳聚糖溶液中,调节pH值使壳聚糖与金属配位.加入H2O2并升高温度对处于配位缔合状态的壳聚糖进行氧化降解,测定了降解产物寡糖的数均相对分子质量和相对分子质量分布.结果表明壳聚糖在与Ca和Zn配位的情况下,可被H2O2迅速降解;降解从大相对分子质量范围开始,降解产物寡糖相对分子质量分布较窄,且聚合度(DP数)越小,寡糖相对分子质量分布指数越小.同时对两者的降解产物进行了清除超氧阴离子自由基(O2-.)的研究,研究发现两者的降解产物均对O2-.有一定的清除作用,清除率随降解产物数均相对分子质量的减小而增大.但用完全降解产物氨基葡糖形成配合物时,清除作用几乎完全消失.
Chitosan was added to the chitosan solution by adjusting the pH value, respectively, to coordinate the chitosan with the metal.Adding H2O2 and increasing the temperature, the chitosan in coordination state was oxidized Degradation and determination of the number average molecular weight and relative molecular mass distribution of degradant oligosaccharides.The results show that chitosan can be quickly degraded by H2O2 in coordination with Ca and Zn.Decomposition starts from the range of large relative molecular mass , The molecular weight distribution of degradation products oligosaccharides is narrow, and the smaller the degree of polymerization (DP), the smaller the molecular weight distribution index of oligosaccharides.At the same time, the degradation products of both products were scavenged by O2- ), The study found that the degradation products of both have a certain scavenging effect on O2-, the clearance rate increases with the decrease of the relative molecular mass of the number of degradation products.But with the complete degradation product glucosamine formation and coordination When the matter is removed almost completely disappeared.