目的:探讨微小RNA-200a-3p(miR-200a-3p)在妊娠期高血压疾病(HDCP)患者胎盘组织中的表达及对滋养细胞生物学行为的影响.方法:收集2017年1月至2019年5月于陕西省西安市第四医院妇产科住院分娩的50例HDCP孕妇(研究组)和50例健康孕妇(对照组)的胎盘组织,采用荧光定量PCR(qPCR)法检测胎盘组织中miR-200a-3p和转甲状腺素蛋白(TTR)mRNA的表达.通过脂质体技术转染miR-200a-3p模拟物(mimic)、抑制物(inhibitor)及阴性对照(NC)至人绒毛膜癌JEG-3细胞,分别采用CCK-8法、Transwell实验以及AnnexinV-FITC/PI实验检测细胞增殖、迁移、侵袭和凋亡.采用生物信息学、双荧光素酶报告基因实验分析miR-200a-3p与TTR的靶向关系,Westren blotting法检测TTR蛋白表达.结果:miR-200a-3p在研究组胎盘组织中的表达显著高于对照组,而TTR mRNA水平显著低于对照组(均P＜0.01).转染miR-200a-3p mimic过表达miR-200a-3p后,可明显促进JEG-3细胞的增殖、侵袭与迁移,并抑制细胞凋亡(P＜0.01).转染miR-200a-3p inhibitor抑制miR-200a-3p表达后,可明显抑制JEG-3细胞的增殖、侵袭与迁移,并促进细胞凋亡(P＜0.01).miR-200a-3p能够明显降低TTR-WT荧光素酶的活性(P＜0.01).转染miR-200a-3p mimic可显著降低TTR蛋白表达水平,而转染miR-200a-3p inhibitor结果与此相反(P＜0.01).结论:miR-200a-3p在HDCP患者胎盘组织中呈高表达,抑制JEG-3细胞中miR-200a-3p表达可抑制细胞的增殖、迁移、侵袭并促进细胞凋亡,miR-200a-3p可能通过负调控TTR参与HDCP的病理机制.“,”Objective:To investigate the expression of mi-croRNA-200a-3p(miR-200a-3p)in placental tissues of pa-tients with hypertensive disorder complicating pregnancy(HD-CP)and its effect on the biological behavior of trophoblasts.Methods:Fifty cases of HDCP(study group)and 50 normal pregnant women(control grouo)in our hospital from January 2017 to May 2019 were collected.The expressions of miR-200a-3p and transthyretin(TTR)mRNA in placental tissues were detected by fluorescent quantitative PCR(qPCR).MiR-200a-3p mimics,inhibitor and negative control were transfect-ed into human chorionic carcinoma JEG-3 cells by liposome.Cell proliferation,migration,invasion and apoptosis were de-tected by CCK-8 method,Transwell test and Annexin V-FITC/PI test,respectively.Bioinformatics and dual luciferase reporter gene experiments were used to analyze the targeting relation-ship between miR-200a-3p.Western blottingwas used to detect the TTR expression.Results:The expression level of miR-200a-3p in study group was significantly higher than that incontrol group,while the expression level of TTR mRNA in study group was significantly lower than that incontrol group(P＜0.01).Transfection of miR-200a-3p mimic to overexpress miR-200a-3p in could significantly promote the proliferation,inva-sion and migration of JEG-3 cells,and inhibit apoptosis(P＜0.01).Transfection of miR-200a-3p inhibitor to inhibit the ex-pression of miR-200a-3p could significantly inhibit the prolif-eration,invasion and migration of JEG-3 cells,and promote apoptosis(P＜0.01).MiR-200a-3p could significantly decrea-se the luciferase activity of TTR-WT(P＜0.01).Transfection of miR-200a-3p mimic could significantly decreased the expres-sion of TTR protein,while the result of transfection of miR-200a-3p inhibitor was on the contrary(P＜0.01).Conclusion:miR-200a-3p is highly expressed in placental tissue of HDCP patients.Inhibition of miR-200a-3p expression in JEG-3 cells can inhibit cell proliferation,migration,invasion,and promote apoptosis.MiR-200a-3p may participate in the pathological mechanism of HDCP through negative regulation of TTR.