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目的探讨5-脂氧合酶激活蛋白基因2354T/A和16699G/A多态性与冠心病的关系。方法采用聚合酶链反应—限制片长多态性技术,对282例经冠状动脉造影证实的冠心病患者和79例对照者进行检测,分析5-脂氧合酶激活蛋白基因2354T/A和16699G/A多态性的基因型和等位基因频率分布情况,检验多态位点与冠心病的关联。结果 5-脂氧合酶激活蛋白基因2354T/A和16699A/G多态性在病例组和对照组均以TT、GG基因型为主。A等位基因为少见型。连锁不平衡分析显示2354T/A,16699G/A间不存在连锁不平衡(D’=0.798,r2=0.037)。单因素分析显示以上2个多态未显示与冠心病的相关性,应用Logistic回归模型将混杂因素矫正后进行分析单个多态位点与疾病的关系时,以上2个多态同样未显示与冠心病的相关性。将以上多态全部纳入构建单体型,有两种单体型频率较高分别为:AG28.6%、TG68.9%,单体型分析显示,两组间比较差异无统计学意义(P>0.05)。结论 5-脂氧合酶激活蛋白基因2354T/A和16699G/A多态性与中国天津汉族人群冠心病易感性无明显关联。
Objective To investigate the relationship between the polymorphisms of 5-lipoxygenase activating protein 2354T / A and 16699G / A and coronary heart disease. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect coronary artery disease in 282 coronary angiography patients and 79 controls. The expressions of 5-lipoxygenase-activating protein 2354T / A and 16699G / A polymorphism genotypes and allele frequency distribution, test polymorphism sites and coronary heart disease. Results The polymorphisms of 5-lipoxygenase activating protein 2354T / A and 16699A / G were mainly genotype TT and GG in both cases and controls. A allele is a rare type. Linkage disequilibrium analysis showed that there was no linkage disequilibrium between 2354T / A and 16699G / A (D ’= 0.798, r2 = 0.037). Univariate analysis showed that the above two polymorphisms did not show correlation with coronary heart disease. Logistic regression model was used to analyze the relationship between single polymorphism and disease after confounding factors. The above two polymorphisms also showed no correlation with coronary heart disease Correlation of heart disease. All the above polymorphisms were all included in the haplotypes of construction. The higher frequencies of the two haplotypes were AG28.6% and TG68.9%, respectively. The haplotype analysis showed that there was no significant difference between the two groups (P > 0.05). Conclusions The polymorphisms of 5-lipoxygenase activating protein 2354T / A and 16699G / A are not significantly associated with susceptibility to coronary heart disease in Han population of Tianjin, China.