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巨细胞病毒(HCMV)血清阳性孕妇宫颈分泌物和尿中常有 HCMV 排出,可能为隐伏病毒再活化所致。已证实孕期 HCMV 特异细胞免疫反应受抑制,但对体液免疫的重要性了解有限,原因是未能确定在防止 HCMV 感染中起重要作用的中和(NT)抗体。过去的研究多以补体结合(CF)、免疫荧光(IFA)及酶联免疫吸附法(ELISA)检测 HCMV 特异抗体,而这些抗体均不同于中和抗体。以空斑形成减少的方法测定中和抗体可探讨孕期保护性体液免疫在 HCMV 感染(再活化)中的作用。
Cytomegalovirus (HCMV) seropositive women with cervical secretions and urinary HCMV often excretion may be due to latent virus reactivation. HCMV-specific cellular immune responses have been shown to be suppressed during pregnancy, but limited understanding of the importance of humoral immunity has not been demonstrated because of the failure to identify neutralizing (NT) antibodies that play an important role in preventing HCMV infection. In the past, many of the HCMV-specific antibodies were detected by complement fixation (CF), immunofluorescence (IFA) and enzyme-linked immunosorbent assay (ELISA), which were different from neutralizing antibodies. Determining neutralizing antibodies with reduced plaque formation may explore the role of protective humoral immunity during pregnancy in HCMV infection (reactivation).