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用无致病性、并能培养的W分枝杆菌生产麻风菌苗作为抗麻风免疫防治的研究已进入Ⅲ期临床试验。本文证明通过控制溶解氧（DO）的水平可以在生物反应器中对W分枝杆菌进行最适化培养,开发了一种商售麻风菌苗的生产工艺。将保存于-70℃Lownstin-Jensen斜面培养基中的W分枝杆菌多次传代后,进行特性、纯度、毒性及免疫原性等检测以作为菌苗候选株。将候选株接种到补充了营养因子的200ml Middlebrook 7H9肉汤中,37℃培养7
With non-pathogenic and can be cultured Mycobacterium leprae production of leprosy vaccine as anti-leprosy immune control research has entered the phase Ⅲ clinical trials. This paper demonstrates that mycobacterium can be optimally cultured in a bioreactor by controlling the level of dissolved oxygen (DO), and a commercial process for producing the leprosy vaccine has been developed. Mycobacterium vaccae stored in Lownstin-Jensen slant culture medium at -70 ℃ was tested for its characteristics, purity, toxicity and immunogenicity after multiple passages. The candidate strain was inoculated into 200 ml of Middlebrook 7H9 broth supplemented with trophic factors and cultured at 37 ° C for 7