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目的构建适合移植使用的BALB/c-nu/nu裸鼠造血微环境损伤模型。方法分别用3.0、4.0、5.0、6.0、7.0Gy剂量辐照BALB/c-nu/nu裸鼠,检测照射后1、3、5、7、14、21 d血象变化,检测照射后1、7、14、21 d基质细胞集落形成单位(CFU-F)和巨核细胞计数,观察照射后骨髓组织活检变化情况。结果7.0 Gy辐照剂量照射后裸鼠全部死亡;6.0 Gy组照射后出现明显骨髓抑制,至照射后21 d不能恢复至照射前水平,且部分裸鼠照射后死亡;3.0、4.0 Gy照射后裸鼠仅出现轻度骨髓抑制;5.0 Gy组照射后出现明显骨髓抑制,未做特殊处理于照射后21 d自行恢复至照射前水平。不同剂量放射线照射后裸鼠CFU-F计数变化不同。3.0 Gy和4.0 Gy组CFU-F在照射后7~14 d出现轻度减少,6.0 Gy组照射后CFU-F明显减少,至照射后21 d不能恢复,5.0 Gy组照射后CFU-F也出现了较明显减少,但在照射后21 d恢复至照射前水平。结论5.0 Gy是BALB/c-nu/nu裸鼠造血微环境损伤模型的适宜照射剂量。
Objective To construct the hematopoietic microenvironment damage model of BALB / c-nu / nu nude mice suitable for transplantation. Methods BALB / c-nu / nu nude mice were irradiated with 3.0, 4.0, 5.0, 6.0 and 7.0Gy doses, respectively, and the changes of blood 1, 3, 5, 7, 14 and 21 d after irradiation were detected. , 14, 21 d of stromal cell colony forming units (CFU-F) and megakaryocyte count, observed changes in the biopsy of bone marrow after irradiation. Results All the nude mice died after irradiation with 7.0 Gy irradiation dose. Myelosuppression was found in the 6.0 Gy group, which could not be restored to the pre-irradiation level 21 days after irradiation, and some nude mice died after irradiation. Mice only showed mild myelosuppression. Myelosuppression was found in 5.0 Gy group, and was restored to the pre-irradiation level 21 days after irradiation without any special treatment. The CFU-F counts of nude mice varied with different doses of radiation. CFU-F in 3.0 Gy and 4.0 Gy groups showed a slight decrease from 7 to 14 days after irradiation, while CFU-F decreased significantly after irradiation in 6.0 Gy group, and could not recover after 21 days of irradiation, CFU-F also appeared after irradiation in 5.0 Gy group The more obvious reduction, but returned to the pre-irradiation level 21 d after irradiation. Conclusion 5.0 Gy is a suitable radiation dose for hematopoietic microenvironment damage model of BALB / c nu nu mice.