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目的探讨羟基磷灰石纳米粒(nHAP)在肝细胞癌中的作用以及可能的机理。方法复习近年来关于nHAP对肝细胞癌作用的文献并进行总结。结果作为一种新型纳米粒子,nHAP主要通过抑制肝癌细胞增殖细胞核抗原(PCNA)和端粒酶基因的表达以及增加细胞内Ca2+浓度,从而抑制肝癌细胞DNA的合成,最终抑制肿瘤细胞的分裂和增殖;另外,nHAP可通过影响肿瘤组织中Paxillin和P130cas的表达、降低肿瘤组织微血管密度、多药耐药基因蛋白和血管内皮生长因子的表达,从而抑制肿瘤细胞的分化、转移;最后,nHAP可通过调节bc-l2及bax蛋白表达,从而诱导肿瘤细胞的凋亡。nHAP与常用化疗栓塞药物联合使用可以增强药效,延长药物作用时间,减少毒副作用。结论 nHAP可抑制肝癌细胞分裂、增殖、分化、转移并促进其凋亡。联合化疗药物使用可增强药效和减少毒副作用。
Objective To investigate the role and possible mechanism of hydroxyapatite nanoparticles (nHAP) in hepatocellular carcinoma. Methods Review the recent literature on the role of nHAP in hepatocellular carcinoma and summarize. Results As a new type of nanoparticle, nHAP inhibits DNA synthesis and ultimately inhibits tumor cell division and proliferation by inhibiting the expression of proliferating cell nuclear antigen (PCNA) and telomerase gene and increasing intracellular Ca2 + concentration in hepatoma cells ; In addition, nHAP can inhibit the differentiation and metastasis of tumor cells by affecting the expression of Paxillin and P130cas in tumor tissues and reducing the expression of microvessel density, multidrug resistance gene protein and vascular endothelial growth factor in tumor tissues. Finally, Regulate the bc-l2 and bax protein expression, thereby inducing tumor cell apoptosis. nHAP and commonly used chemoembolization drugs can enhance efficacy, extend the duration of drug action, reduce side effects. Conclusion nHAP can inhibit hepatocellular carcinoma cell division, proliferation, differentiation, metastasis and promote apoptosis. Combination chemotherapy drugs can enhance efficacy and reduce side effects.