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本文选择49份胃手术标本(包括正常胃粘膜12例、慢性溃疡13例,胃粘膜不典型增生10例和胃管状腺癌14例)为对象,探讨细胞核内DNA含量的变化。采用常规切片,H·E染色、Feulgen反应显示DNA,光镜下分三级,光镜下测核面积,并选新鲜的正常胃粘膜、胃癌各2例应用显微分光光度仪检查。结果发现光镜下各组病变DNA含量分级中,癌>不典型增生>慢性溃疡>正常,具统计学意义;正常胃粘膜和病变细胞核DNA与直径、核面积的平均值,则癌肿表现核大且DNA含量多;正常胃粘膜细胞和病变细胞核DNA含量与核面积的相关,表现DNA Ⅰ级含量与核面积大小呈负相关,DNA Ⅱ、Ⅲ级均呈正相关;显微分光光度仪结果表明,癌细胞的多倍体及不整倍体细胞的百分数占相当数量,其参差不等的结果与镜下所见很吻合。我们认为,细胞化学方法在光镜下测定DNA含量,可以达到半定量的效果。
This article selected 49 gastric surgical specimens (including 12 cases of normal gastric mucosa, 13 cases of chronic ulcers, 10 cases of gastric dysplasia and 14 cases of gastric tubular adenocarcinoma) as subjects to investigate changes in DNA content in the nucleus. The routine sections were stained with H·E staining and Feulgen reaction to show DNA. Light microscopy was performed on three grades. The nuclear area under light microscope was measured. Fresh normal gastric mucosa and 2 cases of gastric cancer were selected and examined by microspectroscopy. The results showed that among the DNA levels of lesions in each group under light microscope, cancer> atypical hyperplasia> chronic ulcer> normal, with statistical significance; normal gastric mucosa and diseased cells, the average nuclear DNA and diameter, nuclear area, then the cancer nuclear expression Large and more DNA content; normal gastric mucosal cells and lesions nuclear DNA content and nuclear area, showing DNA I level content and nuclear area size was negatively correlated, DNA II, III were positively correlated; Microspectrophotometer results show The percentage of polyploid and aneuploid cells in cancer cells accounted for a considerable amount, and their unequal results were in good agreement with what was observed under the microscope. We believe that cytochemical methods can measure DNA content under light microscopy to achieve semi-quantitative results.