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为建立一种能同时鉴别人参、三七、西洋参及其掺杂品的方法。通过分析人参属ITS,18S和matK序列,寻找特异性SNP位点设计引物,建立多重位点特异性PCR法,并对不同来源的人参、三七、西洋参样品进行扩增,根据特异性条带大小进行鉴别。在退火温度为60℃,循环数为35时,人参、三七和西洋参分别出现约250,500,1000 bp的特异性条带。该方法对于人参、三七、西洋参相互掺杂的混合样品,人参中掺杂三七或西洋参以及三七中掺杂西洋参或人参的检出限均为0.5%,对西洋参中掺杂人参的检出限为0.5%,掺杂三七的检出限为1%。表明建立的多重位点特异性PCR可用于人参、西洋参、三七的掺杂鉴定。
In order to establish a method that can simultaneously identify ginseng, notoginseng, American ginseng and their doping products. By analyzing the ITS, 18S and matK sequences of ginseng, searching for specific SNP loci to design primers, establishing multiplex site-specific PCR method, and amplifying samples from different sources of ginseng, notoginseng and American ginseng according to specific bands Size to identify. When the annealing temperature was 60 ℃ and the number of cycles was 35, specific bands of about 250, 500 and 1000 bp appeared in Panax ginseng, Panax notoginseng and Panax quinquefolius. The method for the mixed samples of ginseng, notoginseng, American ginseng mixed with each other, the ginseng doped Panax notoginseng or Panax notoginseng or ginseng ginseng detection limits were 0.5%, ginseng doping ginseng seized The limit of detection is 0.5%, and the detection limit of DADP is 1%. This indicated that the established multiple site-specific PCR could be used to identify the doping of ginseng, American ginseng and notoginseng.