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目的 通过检测外周血淋巴细胞端粒酶活性 ,为癌症的治疗及预后的评估 ,随访提供可靠的依据 .方法 采用PCR- TRAP方法检测癌症患者外周血淋巴细胞中端粒酶活性 .结果 癌症患者 43例中 ,有 37例端粒酶表达阳性 ,总阳性率 86 % ,阳性结果经 EB染色后可见间隔 6 bp的梯形区带 .其中 ,宫颈癌 12例中有 10例阳性表达 ,阳性率 83% ;卵巢癌 6例中有 6例阳性表达 ,阳性率 10 0 % ;肝癌 6例中有 5例阳性表达 ,阳性率 83% ;肺癌 11例中有 9例阳性表达 ,阳性率82 % ;乳腺癌 8例中有 7例阳性表达 ,阳性率 88% ,5例正常对照中未检出阳性者 .结论 端粒酶活性表达与癌症的发生与发展有着密切的关系 ,对癌症患者外周血淋巴细胞端粒酶活性的测定 ,对癌症的治疗、预后的评估 ,随访及复发等都有重要的临床意义 .
Objective To detect the telomerase activity in peripheral blood lymphocytes and provide a reliable basis for cancer treatment and prognosis evaluation.Methods PCR-TRAP was used to detect telomerase activity in peripheral blood lymphocytes of cancer patients. Results Cancer patients 43 In the case, there were 37 cases with positive telomerase expression and a total positive rate of 86%. Positive results showed a trapezoidal interval of 6 bp after EB staining. Among them, 10 of 12 cases of cervical cancer were positive and the positive rate was 83%. Among 6 cases of ovarian cancer, 6 cases had positive expression, and the positive rate was 100%; 6 cases of liver cancer showed positive expression in 5 cases, and the positive rate was 83%; 9 cases of lung cancer had positive expression in 9 cases, the positive rate was 82%; Among the 8 cases, 7 cases were positive and the positive rate was 88%. There were no positive cases detected in 5 normal controls. Conclusion The expression of telomerase activity is closely related to the occurrence and development of cancer, and to the peripheral blood lymphocyte end of cancer patients. Determination of granzyme activity has important clinical significance for cancer treatment, prognosis assessment, follow-up and recurrence.