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目的:探讨TPA对体外培养的人喉鳞状细胞癌细胞株HEp-2细胞的作用。方法:应用不同浓度TPA处理体外培养的HEp-2细胞,MTT法检测HEp-2细胞的存活比率。结果:HEp-2细胞经TPA处理后其细胞增殖能力呈现峰型,高峰出现在10μg/L水平上,此时处理细胞的增殖能力是对照组的1.3~1.4倍。随着TPA浓度的增高,HEp-2细胞的增殖能力逐渐下降。结论:TPA处理可以增强HEp-2细胞的增殖能力。
Objective: To investigate the effect of TPA on human laryngeal squamous cell carcinoma cell line HEp-2. Methods: HEp-2 cells cultured in vitro were treated with different concentrations of TPA. The survival rate of HEp-2 cells was detected by MTT assay. Results: The proliferative ability of HEp-2 cells treated with TPA showed a peak shape with a peak at 10 μg / L. The proliferative capacity of HEp-2 cells was 1.3-1.4 times that of the control group. With the increase of TPA concentration, the proliferation of HEp-2 cells gradually decreased. Conclusion: TPA treatment can enhance the proliferation of HEp-2 cells.