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应用原生质体融合技术,将黑木耳河北6号和野生毛木耳杂交获得的8个融合株,从中筛选出具有黑木耳商品性状,表现有明显的杂交优势,遗传特性稳定的翼杂2号、3号两个新种,经在生产中示范推广,生产性能较好。现将其特性研究报告如下。一、生物学特性 (一)温度将菌株接种于未调pH的PDA培养基斜面试管中心,置于5、10、15、20、25、30、35、40℃温度下培养,6天后测量菌丝生长长度,取日长速平均值(mm/日)。结果表明,翼杂2号菌丝生长起始温度、适宜温度和最适温度分别为15℃、25～35℃和30℃,与亲本河北6号一致,翼杂3号菌丝生长起始温度为20℃,高于亲本株(表1)。
Eight protoplasts obtained from cross between Heilongjiang 6 and wild Mao’ergus were selected by using protoplast fusion technique, and the results showed that they had obvious hybrid vigor and stable genetic characteristics. Two new species, after demonstration in the production promotion, production performance is better. The characteristics of the research report is as follows. First, the biological characteristics (A) temperature The strain was inoculated pH-free PDA medium inclined tube center, placed at 5,10,15,20,25,30,35,40 ℃ temperature culture, measuring bacteria after 6 days Silk growth length, take the day long-speed average (mm / day). The results showed that the initial temperature, optimum temperature and optimum temperature of Yansha 2 mycelia were 15 ℃, 25 ~ 35 ℃ and 30 ℃, respectively, which were consistent with that of the parent Hebei No.6. The growth start temperature 20 ℃, higher than the parent strain (Table 1).