论文部分内容阅读
Objective: To investigate the therapeutic effect of a novel suicide gene system, yeast cytosine deaminase (YCD)/5-Fluorocytosine(5-FC), on P388/DBA murine leukemia model. Methods: P388-YCD-eGFP clone was selected after retrovirus transduction by limited dilution. P388-eGFP and wild type (wt) P388 cells were used as controls. (1) Tumorigenesis study: DBA mice were inoculated intravenously with P388-YCD-eGFP, P388-eGFP or wt P388 cells at a dosage of 5×106 per mouse (n=5). (2) Therapeutic effect study of 5-FC: After inoculation with P388-YCD-eGFP, P388-eGFP or wt P388 cells (n=5), 5-FC was administered at a dosage of 5 μmol/d in each mouse for 2 weeks. 20 μmol/d 5-FC were administered on the 8th day in case 5 μmol/d did not work. The percentage of eGFP+ cells was detected by flow cytometry, frozen section or HE section. Results: Mice in YCD, eGFP and wt P388 group developed leukemia and survived (8.0±1.0) d, (7.6±0.89) d and (7.8±1.64) d (P>0.05), respectively. After being treated with 5-FC for 2 weeks, mice in YCD and eGFP+ groups survived (13.2±1.79) d and (8.4±0.58) d (P