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目的:探讨转染喉癌总RNA的树突状细胞(DCs)诱导抗喉癌反应的免疫效应。方法:用喉癌细胞总RNA转染从健康人外周血诱导的DCs以制备DCs疫苗,MTT法检测其激活的细胞毒T淋巴细胞(CTLs)在体外对喉癌的特异性杀伤作用。将此特异性CTLs预防性接种于裸鼠皮下,7d后接种喉癌细胞,观察其移植瘤的发生率;再将此CTLs治疗性接种于已荷瘤裸鼠皮下(治疗组1次,加强治疗组2次),观察移植瘤的生长情况。结果:喉癌细胞总RNA转染的DCs能在体外激活CTLs,该CTLs能在体外诱导针对喉癌细胞的肿瘤特异性免疫杀伤及抑制作用。在CTLs预防性接种裸鼠后,其移植瘤的发生率明显低于对照组(预防组为33%,对照组为100%,P<0.01);当给荷瘤鼠皮下注射CTLs后,治疗组与加强治疗组移植瘤体积明显小于对照组(P<0.05或P<0.01),且加强治疗组移植瘤体积明显小于治疗组(P<0.05)。结论:转染喉癌总RNA的DCs能在体外活化CTLs,此CTLs不仅在体外能诱导特异性抗喉癌免疫反应,而且能在裸鼠体内抑制移植瘤的发生与生长。
Objective: To investigate the immunological effects of dendritic cells (DCs) transfected with total RNA of laryngeal cancer on anti-laryngeal cancer response. Methods: DCs induced by peripheral blood of healthy human were transfected with total RNA of laryngeal cancer cells to prepare DCs vaccine. MTT assay was used to detect cytotoxic T lymphocytes (CTLs) specific killing effect on laryngeal carcinoma in vitro. The specific CTLs were inoculated subcutaneously in nude mice and laryngeal carcinoma cells were inoculated 7 days later to observe the incidence of xenograft tumors. The CTLs were inoculated subcutaneously into the tumor-bearing nude mice (treatment group 1, intensive treatment Group 2 times) to observe the growth of xenografts. Results: DCs transfected with total RNA of laryngeal carcinoma cells could activate CTLs in vitro. The CTLs can induce tumor-specific immunosuppression and inhibition of laryngeal carcinoma cells in vitro. The incidence of xenografts in nude mice vaccinated with CTLs was significantly lower than that of the control group (33% in the prevention group and 100% in the control group, P <0.01). When CTLs were injected subcutaneously into the nude mice, the treatment group Compared with the control group (P <0.05 or P <0.01), the volume of xenograft tumor in the intensive treatment group was significantly smaller than that in the treatment group (P <0.05). CONCLUSION: DCs transfected with laryngeal cancer total RNA can activate CTLs in vitro. CTLs can not only induce specific anti-laryngeal cancer immune response in vitro, but also inhibit the growth and development of xenografts in nude mice.