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本研究旨在建立骨髓涂片间期荧光原位杂交(I-FISH)的实验方法,为检测多发性骨髓瘤(MM)分子细胞遗传学提供新方法。以骨髓涂片为载体,通过一系列的处理,固定及消化后,用8号染色体着丝粒探针行I-FISH分子细胞遗传学的检测,并比较该方法与常规I-FISH结果的差异。结果表明:两种方法分析非恶性血液病标本的各个信号比例无统计学差异(p>0.05)。骨髓涂片I-FISH研究中,19例MM患者中8例(42.1%)有8号染色体异常,其中8号染色体单体(-8)5例(26.3%),8号染色体三倍体(+8)3例(15.8%)。结论:骨髓涂片I-FISH具有操作简便、经济、准确的特点,可用于MM分子遗传学异常的研究。
This study aimed to establish an experimental method of interphase fluorescence in situ hybridization (I-FISH) for bone marrow smear, and to provide a new method for detecting molecular cytogenetics of multiple myeloma (MM). Using bone marrow smear as carrier, I-FISH molecular cytogenetics was detected by chromosome 8 centromeric probe after a series of treatment, fixation and digestion, and the difference between this method and routine I-FISH was compared . The results showed that there was no significant difference between the two methods in analyzing the proportion of each signal in non-malignant hematological diseases (p> 0.05). In the bone marrow smear I-FISH study, 8 (42.1%) of 19 patients with MM had chromosomal abnormalities of chromosome 8, including 5 (26.3%) on chromosome 8 and a triploid on chromosome 8 +8) 3 cases (15.8%). Conclusion: Bone marrow smear I-FISH has the characteristics of simple, economical and accurate. It can be used in the study of MM molecular abnormality.