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目的 :评价印度鸡骨常山 (Alstoniascholaris)树皮汁对Wistar雄大鼠的抗生育作用。方法 :给雄鼠口服印度鸡骨常山树皮汁 ,每天 2 0 0mg共 60天。生育力和睾丸功能用交配试验、精子活动力、精子密度、生化指标和睾丸细胞动力学等方法来测试。结果 :口服该树皮汁每天 2 0 0mg 60天 ,未见体重下降 ,而睾丸、附睾、精囊和腹前例腺重量明显减少。第19期精子细胞的产生降低了 79.6%。前细线期和粗线期精母细胞的数量分别降低 61.9%和 60 .1%。精原细胞和支持细胞数量也受到影响。曲精细管和间质细胞核面积 ,与对照组相比明显减少 (P<0 .0 1)。精子数和活力的减少引起总的生育力降低。睾丸、附睾、精囊和腹前列腺的蛋白质和唾液酸以及睾丸糖原明显减少。与对照组相比 ,精囊果糖降低 ,睾丸胆固醇升高。用Si凝胶柱色谱分离法取得了以下化合物 :a -香树精 ,b -香树精 ,醋酸lu piol,印度鸭脚树亭 ,热嗪和育亨宾。结论 :所用剂量的印度鸡骨常山树皮汁对雄鼠有明显的抗生育作用。主要的作用环节可能是减数分裂后的生殖细胞( 19期精子细胞 )。
Objective: To evaluate the anti-fertility effects of bark juice of Alstonia scholaris from India on male Wistar rats. Method : The male rats were orally treated with Baked Bark of Changshan Bark, 200 mg daily for 60 days. Fertility and testicular function were tested using mating tests, sperm motility, sperm density, biochemical markers, and testicular cell dynamics. RESULTS: 200 mg/day of bark juice was taken orally, and no weight loss was observed. However, the testis, epididymis, seminal vesicles, and pre-abdominal cases had significantly reduced glandular weight. The 19th generation of sperm cells was reduced by 79.6%. The number of spermatocytes in the previous hairline and fat line decreased by 61.9% and 60.1%, respectively. The number of spermatogonia and supporting cells is also affected. The fine tube and stromal cell nuclear area of the curvilinear tissue were significantly reduced compared with the control group (P < 0.01). Decreased sperm count and vigor result in a reduction in total fertility. The testis, epididymis, seminal vesicles, and ventral prostate have significantly reduced protein and sialic acid as well as testicular glycogen. Compared with the control group, fructose in the seminal vesicles decreased, and cholesterol in the testis increased. The following compounds were obtained by Si-Sepharose column chromatography: a - agarwood, b - agrochemical, lu piol acetate, duck foot tree pavilion, hot azine and yohimbine. Conclusion : The dose of Indian chicken bone from Changshan bark has obvious anti-fertility effects on male rats. The main link may be meiotic germ cells (19 sperm cells).