某些二元酸在吡啶或二甲基甲酰胺(DMF)中以季铵硷滴定,可以得到有二个明显的等当点的N型滴定曲线。另一些酸,在同样情况下得到较不明显的终点的椅型曲线,在低溶剂化效应的媒介中获得最好的结果如吡啶、DMF等,至于所用的硷,可以少量的阳离子(K~+.(CH_2)N~+)和胺的乙醇溶液的滴定剂,更好的是四丁基氢氧化铵的DMF或呲啶溶液。 Certain diacids titrated with quaternary ammonium bases in pyridine or dimethylformamide (DMF) yielded two distinct N-type titration curves at equivalent points. Other acids, in the same case give a less pronounced end point of the chair-type curve, obtaining the best results in media with low solvation effects such as pyridine, DMF, etc. As for the base used, a small amount of cation (K ~ +. (CH 2) N ~ +) and amine in ethanol, more preferably tetrabutylammonium hydroxide in DMF or pyridine solution.