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目的观察妊娠高血压综合征(妊高征)患者胎盘组织血管内皮生长因子(VEGF)和缺氧诱导因子-1α(HIF-1α)的表达情况,探讨两者在妊高征发病中的作用。方法采用免疫组织化学Eli Vision法测定35例妊高征和40例正常产妇的胎盘组织VEGF和HIF-1α表达。结果两者在滋养细胞、血管内皮细胞表达,绒毛间质细胞少量表达。VEGF表达强度轻度妊高征与对照组比较差异无统计学意义,而中度及重度妊高征胎盘与对照组比较差异有统计学意义(P<0.05);妊高征组胎盘HIF-1α的表达高于对照组(P<0.05),且重度高于中度、中度高于轻度。结论妊高征患者胎盘VEGF表达水平降低,而HIF-1α的表达水平升高,均与病情的严重程度相关,共同参与了妊高征的发生和发展过程。
Objective To observe the expression of vascular endothelial growth factor (VEGF) and hypoxia inducible factor-1α (HIF-1α) in placenta of patients with pregnancy induced hypertension (PIH) and to explore their roles in the pathogenesis of PIH. Methods Immunohistochemical Eli Vision method was used to determine the expression of VEGF and HIF-1α in placenta from 35 PIH patients and 40 normal pregnant women. Results Both were expressed in trophoblasts and vascular endothelial cells, with a small amount of villus mesenchymal cells. There was no significant difference in the expression of VEGF between the mild PIH and the control group, while there was significant difference between the placenta of moderate and severe PIH and the control group (P <0.05). The HIF-1α (P <0.05), and were more severe than moderate, moderate and mild. Conclusions The expression of VEGF in the placenta of patients with PIH is decreased, while the expression of HIF-1α is increased. Both of them are related to the severity of the disease and participate in the occurrence and development of PIH.