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目的:建立多波长同时测定地锦草药材中没食子酸、鞣花酸、槲皮素、山柰酚4种指标成分含量的超高效液相色谱法,并比较不同地锦草化学成分的差异。方法:采用Acquity UPLC BEH C_(18)色谱柱(50 mm×2.1 mm,1.7μm),以乙腈-0.2%磷酸水溶液为流动相,梯度洗脱,柱温30℃,流速0.2 mL·min~(-1),检测波长为271 nm(0~2 min)、254 nm(2~6 min)和360 nm(6~8 min)。结果:地锦草中没食子酸、鞣花酸、槲皮素、山柰酚4种成分在8 min内均达到基线分离,线性范围分别为65.88~164.70(R~2=0.999 2)、149.10~372.75(R~2=0.999 2)、27.48~68.70(R~2=0.999 7)、30.24~75.60μg·mL~(-1)(R~2=0.999 2),平均回收率(n=6)分别为99.5%(RSD=1.4%)、98.3%(RSD=0.92%)、99.1%(RSD=1.2%)和99.3%(RSD=1.4%);6批地锦草样品中没食子酸、鞣花酸、槲皮素、山柰酚的含量分别为4.148~7.663、9.727~17.388、1.763~2.977、0.684~2.037 mg·g~(-1)。结论:本文对不同产地地锦草中指标性成分含量差异性的研究,可为地锦草的质量控制提供理论依据。
OBJECTIVE: To establish a method for the simultaneous determination of gallic acid, ellagic acid, quercetin and kaempferol in Eucommia ulmoides by multi-wavelength ultra-performance liquid chromatography (HPLC), and to compare the differences in the chemical constituents of P. diffusa. Methods: The mobile phase was eluted with acetonitrile - 0.2% phosphoric acid in a mobile phase using a Acquity UPLC BEH C 18 column (50 mm × 2.1 mm, 1.7 μm) with a column temperature of 30 ℃ and a flow rate of 0.2 mL · min ~ -1). The detection wavelength was 271 nm (0-2 min), 254 nm (2-6 min) and 360 nm (6-8 min). Results: The contents of gallic acid, ellagic acid, quercetin and kaempferol in Radix Rehmanniae Preparatae reached the baseline within 8 min. The linear range was 65.88 ~ 164.70 (R ~ 2 = 0.999 2), 149.10 ~ 372.75 (R 2 = 0.999 2), 27.48-68.70 (R 2 = 0.999 7), 30.24-75.60 μg · mL -1 (R 2 = 0.999 2) and the average recoveries (n = 6) Were 99.5% (RSD = 1.4%), 98.3% (RSD = 0.92%), 99.1% (RSD = 1.2%) and 99.3% (RSD = 1.4% The content of acid, quercetin and kaempferol were 4.148 ~ 7.663, 9.727 ~ 17.388, 1.763 ~ 2.977, 0.684 ~ 2.037 mg · g ~ (-1) respectively. Conclusion: The study on the difference of the index content of Elymus dahurica in different habitats can provide a theoretical basis for the quality control of Elymus bungeana.