目的探讨星形胶质细胞标志物S100B蛋白浓度变化与七氟烷麻醉躁动中的相关性。方法选择在全麻下行择期白内障手术的患儿30例,美国麻醉医师协会麻醉风险评分(ASA)Ⅰ或Ⅱ级,年龄3~6岁。吸入麻醉诱导为8%七氟烷,待患儿睫毛反射消失后插入喉罩。术中采用3.0%~3.5%的七氟烷维持,调节氧流量2~3 L/min,保持患儿自主呼吸。术毕停止吸入七氟烷,拔除喉罩,记录躁动发生情况。分别于吸入七氟烷前,吸入七氟烷后15 min和术后10 min 3个时间点采集外周静脉血2~3 ml,静置离心后,检测血清S100B蛋白的浓度。结果 30例患儿术前、术中和术后血清S100B蛋白平均浓度分别为(62.76±32.51)、(47.90±22.20)、(48.05±23.53)ng/L,术中和术后浓度值较术前明显降低(P<0.01),但术中和术后二者浓度值的差异无统计学意义(P>0.05)。其中,躁动患儿术中和术后平均血清S100B蛋白浓度均较术前明显下降[(49.71±25.20)ng/L vs.(68.26±39.40)ng/L,(55.59±30.54)ng/L vs.(68.26±39.40)ng/L,P均<0.01],术中和术后的差异无统计学意义(P>0.05);未发生躁动患儿术中和术后平均血清S100B蛋白浓度均较术前明显降低[(46.71±20.64)ng/L vs.(59.11±27.62)ng/L,(43.18±16.95)ng/L vs.(59.11±27.62)ng/L,P均<0.01],术中和术后的差异无统计学意义(P>0.05)。躁动患儿术前、术后S100B蛋白浓度差值与与躁动评分无相关性(r=0.045,P>0.05)。结论 S100B蛋白浓度在麻醉恢复期患儿躁动发生时明显下降,星形胶质细胞并未在躁动过程中表达增强,S100B蛋白浓度的变化与躁动的严重程度无相关性。 Objective To investigate the correlation between the change of S100B protein concentration and the sevoflurane anesthesia agitation in astrocyte. Methods Thirty children undergoing cataract surgery under general anesthesia were anesthetized with American Society of Anesthesiologists anesthesia risk score (ASA) Ⅰ or Ⅱ, aged 3 to 6 years old. Inhaled anesthesia induction of 8% sevoflurane, eyelashes to be children disappear after the insertion of laryngeal mask. Intraoperative use of 3.0% to 3.5% sevoflurane to maintain oxygen flow 2 ~ 3L / min, to maintain spontaneous breathing in children. At the end of surgery, sevoflurane was aspirated, the laryngeal mask was removed, and the occurrence of agitation was recorded. Peripheral venous blood was collected at 3 and 15 min after inhalation of sevoflurane, 15 min after inhalation of sevoflurane, and 10 min after operation respectively. After centrifugation, the concentration of S100B protein in serum was measured. Results The average preoperative, postoperative and postoperative serum concentrations of S100B in the 30 cases were (62.76 ± 32.51), (47.90 ± 22.20) and (48.05 ± 23.53) ng / L, respectively. The intraoperative and postoperative concentrations of S100B protein (P <0.01). However, there was no significant difference between the two groups in intraoperative and postoperative levels (P> 0.05). The average serum S100B protein concentration in patients with agitation was significantly lower than that before operation [(49.71 ± 25.20) ng / L vs (68.26 ± 39.40) ng / L, (55.59 ± 30.54) ng / L vs (68.26 ± 39.40) ng / L, P <0.01, respectively). There was no significant difference between postoperative and postoperative (P> 0.05) (46.71 ± 20.64) ng / L vs. (59.11 ± 27.62) ng / L, (43.18 ± 16.95) ng / L vs. (59.11 ± 27.62) ng / L, P all less than 0.01) There was no significant difference between postoperative and postoperative (P> 0.05). There was no correlation between the preoperative and postoperative S100B protein concentrations and agitation scores (r = 0.045, P> 0.05). Conclusion The concentration of S100B protein in patients with recovery during anesthesia significantly decreased agitation, astrocytes did not increase expression during agitation, S100B protein concentration and the severity of agitation had no correlation.