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利用RAPD分析技术对野生型大麻Ym43及栽培品种大麻Ym36的基因组DNA进行分析,优化了大麻总DNA的提取方法和PCR扩增条件,从Operon公司的OP系列280个随机引物中筛选出42个扩增效果良好的引物,为以后RAPD技术应用于大麻的遗传研究奠定了基础。
The genomic DNA of wild-type cannabis Ym43 and cultivated variety cannabis Ym36 was analyzed by RAPD analysis, and the extraction method of total DNA and PCR amplification conditions were optimized. Forty-two random primers from Operon OP series were screened out The primer with good effect has laid the foundation for the genetic research of applying RAPD technology to cannabis in the future.