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目的:建立桃花的薄层色谱鉴别及高效液相色谱指纹图谱分析方法。方法:采用薄层色谱法对桃花样品中绿原酸、芦丁、金丝桃苷和异槲皮苷进行定性鉴别,以乙酸乙酯-甲酸-水(30∶3∶2)为展开剂,以5%三氯化铝乙醇溶液为显色剂;并采用高效液相色谱法建立桃花的指纹图谱,色谱柱为Sun Fire C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈为流动相A,以0.1%磷酸水溶液为流动相B,线性梯度洗脱[0 min,A-B(14∶86);20 min,A-B(20∶80);40 min,A-B(60∶40);45 min,A-B(14∶86)],流速为1.0 m L·min~(-1),检测波长为353 nm,柱温为30℃;用国家药典委员会“中药色谱指纹图谱相似度评价系统2.0版”处理分析。结果:对11批桃花样品指纹图谱进行评价,确定了18个共有峰,指认了第3、6、7、8号色谱峰分别对应为绿原酸、芦丁、金丝桃苷和异槲皮苷。结论:本研究可用于评价桃花药材质量,为该药材的质量控制提供科学依据。
Objective: To establish a thin layer chromatographic identification of peach and HPLC fingerprint analysis method. Methods: The chlorogenic acid, rutin, hyperoside and isoquercitrin in peach samples were qualitatively identified by thin-layer chromatography. Ethyl acetate-formic acid-water (30: 3: 2) A 5% aluminum trichloride ethanol solution was used as the color reagent. The fingerprints of peach blossom were determined by high performance liquid chromatography (HPLC) on a Sun Fire C_ (18) column (250 mm × 4.6 mm, 5 μm) As mobile phase A, linear gradient elution was carried out using 0.1% phosphoric acid as mobile phase B [0 min, AB (14:86), 20 min, AB (20:80) 45 min, AB (14:86)], the flow rate was 1.0 m L · min -1, the detection wavelength was 353 nm and the column temperature was 30 ℃. Using the National Pharmacopoeia Committee “Chinese Medicines Chromatographic Fingerprint Similarity Evaluation System Version 2.0 ”processing analysis. Results: The fingerprints of 11 batches of peach samples were evaluated, and 18 common peaks were identified. It was confirmed that the peaks No. 3,6,7,8 correspond to chlorogenic acid, rutin, hyperoside and isoquercitrin respectively Glycosides. Conclusion: This study can be used to evaluate the quality of peach medicinal materials and provide a scientific basis for the quality control of the medicinal materials.