目的探讨在选择性深低温(<20℃)条件下,阻断猴脑双侧颈内动脉血流60min后实验动物复苏的可能性及对生命体征、重要内脏器官功能、脑干功能以及脑干形态学的影响。方法分离双侧颈内动、静脉,颈外静脉和腹股沟动、静脉。于右侧颈内动脉插管冷灌注和同侧颈内静脉插管回流静脉血,阻断双侧颈外静脉和左侧颈内动、静脉血流建立脑局部体外循环,通过腹股沟动、静脉插管监测平均动脉压和中心静脉压,根据二者的变化调整冷灌注和静脉血回流的速度。观察脑温变化过程中生命体征的改变,降温前后分析血液生化指标的变化,动物复苏后取脑干行组织病理学检查。结果7只猴均完成选择性深低温阻断脑血流60min实验,其中5只安全复苏。当脑温由37℃降至34.5℃时,出现血压和心率的短暂性增高,随后降低。在深低温期间应用血管活性药物可以维持平均动脉压及心率的稳定,脑温恢复后生命体征逐渐平稳。在血流阻断前后脑干功能无明显变化。血常规(除外白细胞计数)、蛋白质、碱性磷酸酶和胆碱脂酶等项指标在深低温阻断血流前后的变化,差异具有显著性意义(P<0.05或P<0.01);其余各项指标如白细胞计数、转氨酶类、乳酸脱氢酶、肌酸激酶、三酰甘油以及电解质等指标的变化差异无显著性意义(P>0.05)。降温过程中,光学显微镜检查脑干形态和组织 Objective To explore the possibility of resuscitation in experimental animals after blocking bilateral bilateral internal carotid artery blood flow at selective deep hypothermia (<20 ℃) ​​for 60 minutes and to investigate the effects of vital signs, vital organs, brainstem function and brainstem Morphological effects. Methods Bilateral internal carotid artery, vein, external jugular vein and inguinal artery and vein were isolated. In the right side of the internal carotid artery cannulation cold perfusion and ipsilateral internal jugular vein cannulation reflux venous blood, both sides of the external jugular vein and left internal carotid artery and venous blood flow to establish the local cardiopulmonary bypass, the inguinal artery and vein Intubation monitoring of mean arterial pressure and central venous pressure, according to changes in both the adjustment of cold perfusion and venous return speed. The change of vital signs during the change of brain temperature was observed. The changes of blood biochemical indexes were analyzed before and after the temperature was lowered. The pathological changes of the brain stem were observed after the animals recovered. Results All the seven monkeys completed the selective deep hypothermia block cerebral blood flow for 60 minutes, of which 5 were safe. When the brain temperature dropped from 37 ° C to 34.5 ° C, transient increases in blood pressure and heart rate followed by a decrease. The application of vasoactive drugs during deep hypothermia can maintain the stability of mean arterial pressure and heart rate, and the vital signs gradually stabilize after brain temperature recovery. There was no significant change in brainstem function before and after blood flow occlusion. Blood routine (except for leukocyte count), protein, alkaline phosphatase and cholinesterase and other indicators in the blood flow before and after blocking the changes in low temperature, the difference was significant (P <0.05 or P <0.01); the rest of the Indicators such as white blood cell count, aminotransferases, lactate dehydrogenase, creatine kinase, triglyceride and electrolyte were not significantly different (P> 0.05). During cooling, optical microscopy was used to examine brain stem morphology and tissue