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目的为七味白术散与酵母菌的临床联合运用提供微生态学依据。方法给抗生素造模菌群失调腹泻小鼠,分别灌胃25%超微七味白术散[0.04g/(kg·d)]+25%酵母菌[0.25×10~(10)个酵母/(kg·d)]、50%超微七味白术散[0.08g/(kg·d)]、全量传统七味白术散[0.16 g/(kg·d)]、无菌生理盐水0.16g/(kg·d),采集回肠的肠道内容物,提取微生物宏基因组DNA,用乳酸杆菌特异引物PCR扩增后进行ARDRA分析。结果 25%超微七味白术散+25%酵母菌组的OTUs(分类单元)数为5,Shannon指数为2.3219,Brillouin指数为1.3814,与正常组和50%超微七味白术散组数据相同,高于全量传统七味白术散组和模型组;从主成分分析来看,25%超微七味白术散+25%酵母菌组累积贡献率低于正常组,高于50%超微七味白术散组、全量传统七味白术散组和模型组。结论 25%超微七味白术散+25%酵母菌对乳酸杆菌多样性的调控作用优于50%超微七味白术散组和全量传统七味白术散组,更有利于腹泻的治疗。
Objective To provide microbiological basis for the clinical combination of Qiweibaizhu Powder and yeast. Methods The antibiotics were given to the mice with dysbacteriosis diarrhea and fed with 25% superficial Qiwei Baizhu San [0.04g / (kg · d)] + 25% yeast [0.25 × 10 ~ 10 yeast / kg · 50% superfine Qiweibaizhu San [0.08g / (kg · d)], and the total amount of traditional Chinese medicine Baishanbaizhu San [0.16 g / (kg · d)] and sterile saline 0.16g / (kg · d ), Collecting intestinal contents of the ileum, extracting the macro-genomic DNA of the microorganism, carrying out ARDRA analysis after amplification by PCR with the specific primer of Lactobacillus. Results The average number of OTUs (taxonomic units) was 25%, Shannon index was 2.3219, Brillouin index was 1.3814 in 25% superficial Qiwei Baizhu San + 25% yeast group, which was the same as that in normal group and 50% From the perspective of principal component analysis, the cumulative contribution rate of 25% superfine Baishan Baizhu San + 25% yeast group was lower than that of the normal group, higher than that of the 50% superfine Baicaibaizhu San group, The total amount of traditional Baishasai Baizhu powder group and model group. Conclusions 25% superficial Qiwei Baizhu San + 25% yeasts have a better regulation on the diversity of Lactobacillus than 50% superficial Qiwei Baizhu San and full traditional Qiwei Baizhu San, more conducive to the treatment of diarrhea.