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目的:研究健脑益智胶囊对颅脑损伤后大鼠血清NSE含量的影响。方法:将SD大鼠按照随机原则分成4组,即:A组(假手术组),B组(模型对照组),C组(吡拉西坦治疗组),D组(健脑益智胶囊治疗组);每组再按照标本采集时间随机分为4个亚组(即A1、A2、A3、A4;B1、B2、B3、B4;C1、C2、C3、C4;D1、D2、D3、D4),每组10只。采用自由落体方法造模后,C组给予吡拉西坦治疗,D组给予健脑益智胶囊治疗,造模后第1d、3d、5d、7d、10d观察大鼠一般状况(精神状态、进食量、行为活动度)和血清NSE的含量变化并进行分析。结果:颅脑损伤后大鼠血清NSE水平较假手术组明显升高(P<0.01),受伤后大鼠血清NSE水平随时间的推移逐渐降低;C和D组NSE下降水平较明显;第7d,D组血清NSE水平较B组和C组明显降低,有显著差异(b、c:P<0.05),接近A组,两组无明显差异(a:P>0.05);第10d,C组和D组血清NSE水平较B组明显降低,两组间有明显差异(f、g:P<0.05),接近A组,两组间无明显差异(d、e:P>0.05)。结论:健脑益智胶囊在受伤后能促进TBI后大鼠血清NSE含量的降低,改善TBI后症状,可能与其能减轻脑组织及神经细胞的继发性损伤有关。
Objective: To study the effect of Jiannao Yizhi Capsule on serum NSE in rats after traumatic brain injury. Methods: The SD rats were randomly divided into 4 groups according to randomization: group A (sham operation group), group B (model control group), group C (piracetam treatment group), group D Treatment group). Each group was randomly divided into four subgroups according to the specimen collection time (ie A1, A2, A3, A4; B1, B2, B3, B4; C1, C2, C3, C4; D1, D2, D3, D4), 10 in each group. After modeling with free-fall method, group C was treated with piracetam, group D was treated with jiannaoyizhi capsule. The rats were observed on the 1st, 3d, 5d, 7d and 10d after the model was established (mental state, eating Volume, activity level) and serum NSE levels were analyzed and analyzed. Results: Serum levels of NSE in traumatic brain injury rats were significantly higher than those in sham-operation group (P <0.01). NSE levels of rats in traumatic brain injury gradually decreased with the passage of time. NSE decreased significantly in groups C and D. (P <0.05). The level of serum NSE in group D was significantly lower than that in group B and group C (P <0.05). There was no significant difference between group A and group A (P <0.05). There was no significant difference between the two groups (d, e: P> 0.05). CONCLUSION: Jiannao Yizhi capsule can promote the decrease of serum NSE level and improve the symptoms after TBI in rats after TBI, which may be related to its ability to reduce secondary injury of brain tissue and nerve cells.