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目的通过检测在不同氧浓度条件下培养的正常胎盘组织及妊娠期高血压疾病(hypertensive disorders of pregnancy,HDP)胎盘组织中可溶性血管内皮生长因子受体-1(soluble fms-like tyrosine kinase-1,sFlt-1)的分泌情况,从而了解缺氧因素对sFlt-1分泌的影响。方法将正常胎盘组织及HDP胎盘组织在正常氧浓度(20%氧浓度)及低氧浓度(1%氧浓度)条件下进行培养,通过检测人绒毛膜促性腺激素(human chorionic gonadotrophin,HCG)浓度了解胎盘组织分泌状态,从而筛选出培养生长良好的胎盘组织进行sFlt-1浓度检测,比较正常胎盘组织及HDP胎盘组织在正常氧浓度(20%氧浓度)及低氧浓度(1%氧浓度)条件的分泌浓度。结果对正常胎盘,正常氧浓度下第4天及第5天sFlt-1浓度分泌无差异,缺氧条件下sFlt-1分泌受抑制;对HDP胎盘,正常氧浓度下第5天较第4天sFlt-1分泌持续增加,缺氧条件下sFlt-1分泌受抑制。结论 HDP胎盘组织sFlt-1分泌的负反馈机制失灵;缺氧对正常或HDP胎盘组织的sFlt-1分泌起到抑制作用。
OBJECTIVE: To detect soluble fms-like tyrosine kinase-1 (MMP-2) in placental tissue of normal placenta and hypertensive disorders of pregnancy (HDP) sFlt-1) secretion in order to understand the impact of hypoxia on sFlt-1 secretion. Methods Normal placenta and placenta accreta HDP were cultured under the conditions of normal oxygen concentration (20% oxygen concentration) and low oxygen concentration (1% oxygen concentration). By detecting the concentration of human chorionic gonadotrophin (HCG) (1% oxygen concentration), normal placenta tissue and placenta accreta HDP placenta in normal oxygen concentration (20% oxygen concentration) and low oxygen concentration (1% oxygen concentration) Secretion of conditions. Results In normal placenta, there was no difference in sFlt-1 concentration on day 4 and day 5 under normal oxygen concentration, and sFlt-1 secretion was inhibited under hypoxia. For HDP placenta, sFlt-1 secretion continued to increase, sFlt-1 secretion was inhibited under hypoxic conditions. Conclusion The negative feedback mechanism of sFlt-1 secretion in HDP placenta fails; hypoxia can inhibit the secretion of sFlt-1 in normal or HDP placenta.