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目的:探讨卵巢癌血管生成拟态的临床意义及血管生成拟态与缺氧诱导因子H IF-1α、基质金属蛋白酶MMP-2表达的关系。方法:收集南通大学附属医院2000年1月~2009年5月间经手术切除的卵巢肿瘤组织石蜡标本81例,采用CD34和PAS双重染色方法证实卵巢癌组织中存在VM,探讨卵巢癌组织中VM与卵巢癌组织类型、病理分级、临床分期、肿瘤指标、患者生存时间的相关性,及VM与H IF-1α、MMP-2表达的关系。结果:卵巢癌中出现VM的几率要明显高于交界性卵巢肿瘤;低分化卵巢癌VM发生的几率要高于高分化的卵巢癌;Kaplan-Meier生存分析提示卵巢癌中存在VM的患者生存时间比无VM患者短。VM与H IF-1α蛋白、MMP-2蛋白表达相关。结论:具有血管生成拟态的卵巢癌组织分化低,患者临床预后差;H IF-1α、MMP-2表达与卵巢癌血管生成拟态密切相关。
Objective: To investigate the clinical significance of vasculogenic mimicry and the relationship between angiogenic mimicry and hypoxia-inducible factor HIF-1α and MMP-2 in ovarian cancer. Methods: Totally 81 paraffin-embedded specimens of ovarian tumors were collected from January 2000 to May 2009 in the Affiliated Hospital of Nantong University. VMs in ovarian cancer tissues were confirmed by double staining of CD34 and PAS, The correlation between the expression of VM and HIF-1α, MMP-2 in ovarian cancer tissues, pathological grade, clinical stage, tumor index and patient’s survival time were studied. Results: The incidence of VM in ovarian cancer was significantly higher than that in borderline ovarian cancer. The incidence of VM in poorly differentiated ovarian cancer was higher than that in well-differentiated ovarian cancer. Kaplan-Meier survival analysis suggested survival time of patients with VM in ovarian cancer Shorter than patients without VM. VM is associated with HIF-1α protein and MMP-2 protein expression. CONCLUSION: Ovarian cancer with low angiogenic mimicry has poor differentiation and poor clinical prognosis. The expression of HIF-1α and MMP-2 is closely related to the vasculogenic mimicry of ovarian cancer.