论文部分内容阅读
目的 探讨反义HSP 90降低HeLa细胞恶性度的机制 .方法 经荧光染色观察细胞形态 ,DNALadder及流式细胞仪检测凋亡细胞 .结果 转染有反义HSP 90的HeLa细胞经荧光染色细胞呈不均一亮蓝色 ,荧光染色观察可见细胞变小 ,染色质浓缩并产生凋亡小体 ,DNALadder可见明显的梯形条带 ,流式细胞仪检测有凋亡的细胞 .结论 反义HSP 90可诱导细胞凋亡 .
Objective To investigate the mechanism of antisense HSP90 in reducing the malignancy of HeLa cells.Methods The morphological changes of the cells were observed by fluorescence staining and the apoptotic cells were detected by DNALadder and flow cytometry.Results The transfected HeLa cells transfected with antisense HSP90 cells showed no fluorescence staining The cells were bright blue, the cells became smaller, the chromatin condensed and the apoptotic bodies were observed, the obvious trapezoidal bands were observed in DNALadder and the apoptotic cells were detected by flow cytometry.Conclusion Antisense HSP90 can induce cells Apoptosis.