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目的:探讨丝裂原活化蛋白激酶的激酶MKK4基因启动子区-1304T/G多态性与散发性鼻咽癌遗传易感性的关系。方法:采用聚合酶链反应-限制性片段长度多态性法检测30例健康人和90例鼻咽癌患者的MKK4基因-1304T/G位点单核苷酸多态性。结果:鼻咽癌患者TG+GG基因型的频率明显高于对照人群(82.2%vs 66.7%,χ~2=10.076,P<0.05),与-1304TT基因型相比,-1304TG杂合子患鼻咽癌的风险降低了0.56倍(95%CI=0.164-1.178,P<0.01),-1304GG型降低了0.58倍(95%CI=0.126-1.381,P<0.01),变异基因型TG+GG患鼻咽癌的风险降低了0.72倍(95%CI=0.105-0.753,P<0.01)。结论:MKK4基因-1304T/G基因多态性与鼻咽癌发病风险有关,-1304G变异能降低鼻咽癌患病风险,是散发性鼻咽癌的独立保护因素。
Objective: To investigate the relationship between the -1304T / G polymorphism of MKK4 gene promoter and susceptibility to sporadic NPC in mitogen-activated protein kinase (PKC). Methods: Single nucleotide polymorphism (SNP) -1304T / G of MKK4 gene was detected in 30 healthy people and 90 patients with nasopharyngeal carcinoma by polymerase chain reaction-restriction fragment length polymorphism. Results: Compared with the -1304TT genotype, the frequency of TG + GG genotypes in nasopharyngeal carcinoma patients was significantly higher than that in control subjects (82.2% vs 66.7%, χ ~ 2 = 10.076, P <0.05) The risk of pharyngeal cancer was reduced by 0.56-fold (95% CI = 0.164-1.178, P <0.01) and -1304GG by 0.58-fold (95% CI 0.126-1.381, P <0.01) The risk of NPC was reduced by 0.72-fold (95% CI = 0.105-0.753, P <0.01). CONCLUSION: The -1304T / G gene polymorphism of MKK4 gene is associated with the risk of nasopharyngeal carcinoma. The -1304G mutation can reduce the risk of nasopharyngeal carcinoma and is an independent protective factor for sporadic nasopharyngeal carcinoma.