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In this paper, a double artificial neural network (DANN) algorithm was used to parse near infrared (NIR) reflectance spectrum of Cofrel medicines. The contents of benproperine phosphate, which is the effective ingredient in Cofrel medicines, were accurately nondestructive quantitatively predicted. Compared the results with those of HPLC, the relative errors (RE %)were less than 0.18%. The analytical results could be applied to qualitative control of Cofrel medicines.