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利用高荧光效率的石墨烯量子点(GQDs)作为传感元件,设计组装GQDs脂肪酶活荧光传感器。当Hg2+存在时,Hg2+通过静电吸引作用吸附在GQDs表面,导致GQDs荧光被猝灭;加入脂肪酶及其底物巯基乙酸甲酯(MT),脂肪酶能够水解其底物生成巯基乙酸(TGA),TGA与Hg2+之间具有更强的结合力,从而使Hg2+从GQDs表面释放出来,GQDs的荧光又重新恢复,且荧光恢复强度与脂肪酶的活性有关,因此可以利用此荧光传感策略来定量检测脂肪酶活。在最优条件下,测得的脂肪酶检出线性范围为0.05~1.6 mg/m L,检出限为2.77×10-4 mg/m L。该方法还测定了另外四种商品化脂肪酶的活性,实验结果与恒电位滴定法测定结果一致,证明该方法具有良好的实用性。此外,与传统的比色法相比,该方法具有更高的灵敏度和选择性,简单方便且可用于高通量、实时检测脂肪酶活性。
Using GQDs with high fluorescence efficiency as sensing elements, GQDs lipase active fluorescence sensors were designed and assembled. When Hg2 + is present, Hg2 + is adsorbed on the surface of GQDs by electrostatic attraction, resulting in quenching of GQDs fluorescence. Adding lipase and its substrate, methyl thioacetate (MT), lipase hydrolyzes its substrate to produce thioglycolic acid (TGA) , TGA and Hg2 + have stronger binding force, so that Hg2 + release from the surface of GQDs, GQDs fluorescence and resumed, and the fluorescence recovery intensity and the activity of lipase, so you can use this fluorescence sensing strategy to quantify Detection of lipase activity. Under the optimal conditions, the linear range of lipase was 0.05-1.6 mg / mL and the limit of detection was 2.77 × 10-4 mg / mL. The method also determined the activity of four other commercially available lipases. The experimental results are consistent with the results of potentiostatic titration, which proves that the method has good practicability. In addition, compared with the traditional colorimetric method, this method has higher sensitivity and selectivity, simple and convenient and can be used for high-throughput, real-time detection of lipase activity.