论文部分内容阅读
目的 探讨多药耐药 (multidrugresistance ,MDR )基因及其编码的糖蛋白 (P glycoprotein ,P gp)在结直肠癌中的表达及其临床意义。方法 采用逆转录 聚合酶链式反应 (RT PCR )方法定量分析mdr1基因在不同分化程度、不同Duke′s分期的癌组织、癌旁组织和正常组织中的表达量 ;应用免疫组化方法检测相应的P gp表达率。 结果 大肠癌组织、癌旁组织与正常组织的mdr1基因和P gp表达水平有非常显著性差异 (P <0 .0 1) ;高、中分化与低、未分化癌相比 ,差异非常显著 (P <0 .0 1) ;不同性别、不同肿瘤的生长部位及Duke′s分期 ,其mdr1的表达无显著性差异 (P >0 .0 5 )。结论 大肠癌组织存在内源性MDR。检测MDR基因表达可以预测患者对化疗药物的敏感性 ,从而使化疗个体化。
Objective To investigate the expression of multidrug resistance (MDR) gene and its encoding glycoprotein (P gp) in colorectal cancer and its clinical significance. Methods RT-PCR was used to quantitatively analyze the expression of mdr1 gene in different degree of differentiation, Duke’s staging cancer tissues, adjacent tissues and normal tissues. The expression of mdr1 gene was detected by immunohistochemistry Of P gp expression rate. Results The expressions of mdr1 gene and P gp in colorectal cancer tissues, adjacent non-cancerous tissues and normal tissues were significantly different (P <0.01). There was a significant difference between high and moderately differentiated tumors and low undifferentiated carcinoma P <0.01). There was no significant difference in the expression of mdr1 between different sex, different tumor growth sites and Duke’s stage (P> 0.05). Conclusion There are endogenous MDR in colorectal cancer. Detection of MDR gene expression can predict the patient’s sensitivity to chemotherapeutic drugs, thus making the chemotherapy individualized.