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目的了解北京市昌平区农村饮用水中铜绿假单胞菌耐药谱分布、致病性及分子流行病学特征,为该菌污染生活饮用水而引起介水传染病暴发及流行的防控提供参考。方法按照GB/T 8538 2008进行菌株分离和鉴定,并对菌株进行药物敏感性试验、毒力基因检测及脉冲场凝胶电泳分型(PFGE)。结果 36株铜绿假单胞菌多分离于出厂水(27株),对哌拉西林(PIP)、哌拉西林/他唑巴坦(TZP)、头孢他啶(CAZ)、头孢吡肟(FEP)、氨曲南(ATM)、亚胺培南(IPM)、美罗培南(MEM)、阿米卡星(AN)、庆大霉素(GM)、妥布霉素(TM)、环丙沙星(CIP)、左氧氟沙星(LEV)12种抗生素完全敏感。36株铜绿假单胞菌共有4种毒力基因谱(T1~T4),T1型(las I、lasR、rhl I、rhlR、tox A、plc H、rhl AB、las B)为主要毒力基因谱(25株,69.44%)。36株菌有11个PFGE型别,其中0003、0010为本地区优势PFGE型别。结论昌平区农村生活饮用水中铜绿假单胞菌主要分离于出厂水,无耐药性,主要PFGE型别为0003和0010,主要毒力基因谱为T1型。
Objective To understand the drug resistance profile, pathogenicity and molecular epidemiology of Pseudomonas aeruginosa in rural drinking water in Changping District of Beijing. To provide prevention and control of outbreak of water-borne infectious disease and epidemic for the bacteria to contaminate domestic drinking water reference. Methods The strains were isolated and identified according to GB / T 8538 2008. The strains were tested for drug susceptibility, virulence genes and pulsed field gel electrophoresis (PFGE). Results Totally 36 strains of Pseudomonas aeruginosa were isolated from the factory water (27 strains), and the isolates of piperacillin (PIP), piperacillin / tazobactam (TZP), ceftazidime (CAZ), cefepime (FEP) Aztreonam, imipenem (IPM), meropenem (MEM), amikacin (AN), gentamicin (GM), tobramycin (TM), ciprofloxacin CIP), Levofloxacin (LEV) 12 kinds of antibiotics are completely sensitive. There were four virulence gene profiles (T1 ~ T4) in 36 isolates of Pseudomonas aeruginosa, and T1 (lasR, rhl I, rhlR, toxA, plc H, rhl AB, Spectrum (25 strains, 69.44%). 36 strains had 11 PFGE types, of which 0003,0010 belonged to the predominant PFGE type in this area. Conclusion Pseudomonas aeruginosa in rural drinking water in Changping district is mainly isolated from the factory water and has no drug resistance. The main PFGE types are 0003 and 0010, and the major virulence gene spectrum is T1 type.