由于缺少有效的药物,犬弓首线虫幼虫引起的内脏幼虫移行症很难治愈。作者曾研制了一种具有较强杀幼虫效果的药物—己基2,4-二羟基苯甲酸盐(HDB),并用相对迁移指数(RM)和染料清除指数(DE)来评价对幼虫的杀伤力。但由于RM和DE测试的结果之间存在着明显的差异,因而作者试用四氮唑盐(MTT)染剂试验评价驱蠕虫药对体外培养的犬弓首线虫二期幼虫的杀伤效果。 试验用幼虫为培养不到20周的二期幼虫,存在于幼虫活细胞线粒体内的细胞色素C_1酶可利用MTT,使黄色的水溶性MTT Due to the lack of effective drugs, visceral larva migrans caused by canine nematode larvae are difficult to cure. The authors have developed a hexyl 2,4-dihydroxybenzoate (HDB), a stronger larvicidal drug, and evaluated killing of larvae using relative migration index (RM) and dye removal index (DE) force. However, due to the significant difference between the results of RM and DE tests, the authors attempted to evaluate the killing effect of repellent on the second stage larvae of T. gondii by MTT assay. The larvae tested were secondary larvae cultured for less than 20 weeks, and cytochrome C 1 enzymes present in mitochondria of live larvae could utilize MTT to render yellow, water-soluble MTT