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[目的]研究迷迭香水提液对大鼠脑缺血再灌注损伤后超氧化物歧化酶(SOD)活力和羟自由基(.OH)抑制能力的影响。[方法]取健康SD雌性大鼠50只,采用4-血管阻断(4-VO)方法建立全脑缺血再灌注动物模型,造模前灌胃给药7 d,造模后3 h取材,利用分光光度法检测大鼠肝、脑组织中超氧化物歧化酶(SOD)活力和羟自由基(.OH)抑制能力。[结果]与模型组比较,迷迭香水提液各剂量组均能显著提高大鼠各组织中SOD活力(P<0.01)和.OH抑制能力(P<0.05或P<0.01),并存在一定的量效关系,以高剂量组(50 g/kg)效果最佳。[结论]迷迭香水提液对脑缺血再灌注损伤有保护作用,其机制可能与改善氧化应激状态有关。
[Objective] To investigate the effects of aqueous extract of rosemary on the activities of superoxide dismutase (SOD) and hydroxyl radical (.OH) after cerebral ischemia-reperfusion injury in rats. [Methods] Fifty SD female rats were randomly divided into four groups. The model of global cerebral ischemia / reperfusion was established by 4-Vascular occlusion (4-VO) The superoxide dismutase (SOD) activity and hydroxyl radical (.OH) inhibitory activity in rat liver and brain were detected by spectrophotometry. [Result] Compared with the model group, each dose group of rosemary aqueous extract could significantly increase the SOD activity (P <0.01) and the inhibitory effect of .OH (P <0.05 or P <0.01), and there was a certain Dose-effect relationship, with high-dose group (50 g / kg) the best effect. [Conclusion] The aqueous extract of rosemary has a protective effect on cerebral ischemia-reperfusion injury, and its mechanism may be related to the improvement of oxidative stress.