本文在研究了人白细胞介素6(IL-6)基因转染瘤苗对白血病小鼠体内治疗作用及其部分免疫机理的基础上,进一步观察了IL-6基因转染瘤苗治疗白血病小鼠后产生细胞因子(包括IL-1,IL-2,IFN-γ,TNF及GM-CSF)水平的变化。结果表明,IL-6基因转染瘤苗在体内对小鼠脾淋巴细胞或腹腔巨噬细胞(Mφ)经诱导后分泌的上述细胞因子(除IFN-γ外)各组均有不同程度的促进作用。以此种新型瘤苗辅以低剂量IL-2和低剂量环磷酰胺(Cy)治疗后促进作用最为明显。从而为IL-6基因转染瘤苗治疗白血病的免疫学机理提出了新的认识。 Based on the study of therapeutic effect and partial immune mechanism of human interleukin 6 (IL-6) gene transfection tumor vaccine on leukemia mice in vivo, we further observed that IL-6 gene transfection tumor vaccine can treat leukemia mice (Including IL-1, IL-2, IFN-γ, TNF and GM-CSF) levels. The results showed that IL-6 gene transfected vaccine in vitro stimulated the splenic lymphocytes or peritoneal macrophages (Mφ) secreted by the above cytokines (except IFN-γ) in vitro to varying degrees effect. This new type of tumor vaccine supplemented with low-dose IL-2 and low-dose cyclophosphamide (Cy) after the promotion of the most obvious role. Therefore, a new understanding of the immunological mechanism of IL-6 gene transfection tumor vaccine for the treatment of leukemia has been proposed.