【摘 要】
：
采用PCR技术从藏系绵羊皮肤组织细胞中扩增出了血管内皮细胞生长因子(VEGF)基因,长度为573 bp,共编码190个氨基酸；序列比对发现,VEGF基因在所选择的多个物种之间具有高度同源
【机 构】
：
青海大学农牧学院动物医学系,西南民族大学生命科学与技术学院,沈阳农业大学畜牧兽医学院,青海大学畜牧兽医科学院
论文部分内容阅读
采用PCR技术从藏系绵羊皮肤组织细胞中扩增出了血管内皮细胞生长因子(VEGF)基因,长度为573 bp,共编码190个氨基酸；序列比对发现,VEGF基因在所选择的多个物种之间具有高度同源性,相似性在100％～ 74.5％之间,其中藏系绵羊与普通绵羊的VEGF基因序列完全相同,其次为牛和猪,相似性分别为99.1％和96:2％,而与鸡的同源性最低,相似性为74.5％.采用实时荧光定量PCR SYBR Green Ⅰ荧光染料法,对藏系绵羊皮肤组织细胞中VEGF基因的表达水平进行了相对定量分析,建立了快速检测VEGF基因表达量的方法.检测结果表明:给妊娠后期藏系母羊补饲自制的营养舔砖和颗粒饲料后,所产羔羊体内VEGF基因的表达水平分别为对照组的11.5倍和0.85倍.为提高羔羊皮肤组织中VEGF基因mRNA的表达水平,对妊娠后期藏系母羊补饲自制的营养舔砖,效果优于补饲颗粒饲料.
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