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寒地冬小麦膨胀素(Expansin)基因的表达特性同根系建成密切相关,而形成发达的根系是高寒地区冬小麦成功越冬返青的关键。为明确低温对高寒地区冬小麦膨胀素基因表达特性的影响,进而提高冬小麦对寒冷的适应性,以高抗寒冬小麦东农冬麦1号为试验材料,以正常冬小麦济麦22号为对照材料,运用实时荧光定量PCR技术对经不同低温处理的小麦幼苗根组织中编码α-expansin(EXPA)的基因TaEXPA5、TaEXPA6和TaEXPA7的表达特性进行分析。结果表明,在4℃、-10℃和-20℃低温处理下,高抗寒冬小麦东农冬麦1号中TaEXPA5、TaEXPA6和TaEXPA7基因的相对表达量与对照相比呈现显著性上升,其中,4℃处理条件下,TaEXPA6和TaEXPA7基因的相对表达量变化幅度较明显,-10℃处理条件下,TaEXPA5基因的相对表达量变化幅度较明显,-20℃处理条件下,TaEXPA5和TaEXPA7基因的相对表达量变化幅度较明显。说明低温影响小麦根组织中TaEXPA5、TaEXPA6和TaEXPA7基因的表达,三个基因与抗御低温胁迫具有相关性,并且各基因表达存在一定的协作性。
The expression characteristics of Expansin gene in winter wheat are closely related to the establishment of root system, while the formation of developed root system is the key to successful winter wheat regreening in the alpine region. In order to clarify the effect of low temperature on swollenin gene expression in alpine region and to improve the adaptability of winter wheat to cold, winter wheat Dongnong Dongmai 1 with high cold-resistance was used as experimental material and normal winter wheat, The expression characteristics of TaEXPA5, TaEXPA6 and TaEXPA7 genes encoding α-expansin (EXPA) in root tissues of wheat seedlings under different low temperature treatments were analyzed by real-time fluorescence quantitative PCR. The results showed that the relative expression levels of TaEXPA5, TaEXPA6 and TaEXPA7 genes in Dongnong Dongmai 1 with high cold-tolerant winter wheat increased significantly at 4 ℃, -10 ℃ and -20 ℃. Among them, The relative expression level of TaEXPA6 and TaEXPA7 was more obvious under the treatment of 4 ℃, and the relative expression of TaEXPA5 was more obvious under the treatment of -10 ℃. The relative expression of TaEXPA5 and TaEXPA7 under the condition of -20 ℃ The amount of expression changes more obvious. The results showed that low temperature affected the expression of TaEXPA5, TaEXPA6 and TaEXPA7 genes in wheat roots. The three genes were correlated with the resistance to low temperature stress, and the expression of each gene had some cooperation.