论文部分内容阅读
实验主要利用花粉管通道技术将淀粉分支酶基因的反义表达载体转入玉米自交系中,同时探讨了不同导入时间、不同DNA浓度、不同导入量等对其转化率的影响,并从中得出花粉管通道法的最佳转化条件。同时将转化的玉米植株进行PCR检测,初步证明外源目的基因已整合到玉米基因组中,并得到了转化植株的种子。
The experiment mainly used the pollen tube channel technology to transfer the antisense expression vector of starch branching enzyme gene into maize inbred line, and discussed the influence of different introduction time, different DNA concentration and different introduction amount on its conversion rate Out of the best pollen tube channel conditions of conversion. At the same time, the transformed corn plants were detected by PCR, and it was initially proved that the exogenous gene of interest had been integrated into the genome of the corn and the seeds of the transformed plants were obtained.