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目的:探讨化疗联合细胞因子诱导杀伤(cytokine-induced killer,CIK)细胞输注对非霍奇金淋巴瘤(non-Hodgkinlymphoma,NHL)患者血清中细胞因子的影响。方法:用ELISA法检测33例患者化疗前、后及CIK细胞输注后血清中对肿瘤血管生长有促进作用的细胞因子血管内皮生长因子(VEGF)和转化生长因子β(TGF-β)以及对机体免疫系统起积极调节作用的细胞因子白细胞介素-2(IL-2)和γ-干扰素(IFN-γ)的浓度。结果:与健康对照组比较,NHL患者血清中VEGF和TGF-β的浓度明显升高(P<0.05),而IL-2和INF-γ的浓度均下降(P<0.05)。化疗后患者VEGF和TGF-β的浓度明显下降(P<0.05),IL-2和INF-γ的浓度无明显改变(P>0.05),但经CIK细胞输注治疗后,患者外周VEGF和TGF-β的浓度没有进一步改善(P>0.05),而IL-2和INF-γ明显升高(P<0.05)。结论:NHL患者存在免疫功能异常,体内细胞因子紊乱,化疗减少肿瘤负荷,但对机体免疫系统造成损害。而经CIK细胞输注后机体抗肿瘤免疫能力增强。因此,化疗联合CIK细胞输注治疗可提高NHL患者的免疫功能,改善患者生存质量,对NHL患者的治疗具有潜在价值。
Objective: To investigate the effects of chemokine combined with cytokine-induced killer (CIK) infusion on cytokines in patients with non-Hodgkin lymphoma (NHL). Methods: The serum levels of VEGF, TGF-β and TGF-β in sera of 33 patients before and after transfusion of CIK cells and their effect on tumor angiogenesis were detected by ELISA. The body’s immune system actively regulates the concentrations of cytokines interleukin-2 (IL-2) and interferon-gamma (IFN-γ). Results: Compared with the healthy control group, the concentrations of VEGF and TGF-β in serum of patients with NHL were significantly increased (P <0.05), while the concentrations of IL-2 and INF-γ were decreased (P <0.05). After chemotherapy, the concentrations of VEGF and TGF-β in the patients were significantly decreased (P <0.05), while the concentrations of IL-2 and INF-γ in the patients did not change significantly (P> 0.05) (P> 0.05), while the levels of IL-2 and INF-γ were significantly increased (P <0.05). Conclusion: NHL patients have abnormal immune function, cytokines in vivo, chemotherapy reduce tumor burden, but cause damage to the body’s immune system. The CIK cells after infusion of anti-tumor immune capacity increased. Therefore, chemotherapy combined with CIK cell infusion therapy can improve the immune function of patients with NHL, improve the quality of life of patients with NHL patients with potential value.