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甲基异柳磷系华中师范大学农药所合成的一种新型有机磷杀虫剂。我们在对其系统毒理学研究的基础上,和用离体肝脏为模型,对甲基异柳磷在肝内的代谢进行了初步探讨。 一、材料与方法 1.动物:本室繁殖的纯系Wistar雄性大鼠(230～250g)14只,随机分为两组,每组7只,对照组为进行离体肝脏灌流组,实验组在离体灌流30分钟后.将甲基异柳磷(21.52mg/kg体重)灌流于介质中。 2.离体肝脏灌流:灌流系统参照张滋法自制,灌流介质为含有0.15％葡萄塘的Krebs-Rin-ger碳酸氢盐缓冲液,pH7.0～7.2。手术操作要
Methyl isoflurane is a new kind of organophosphorus insecticide synthesized by pesticide of Huazhong Normal University. Based on the systematic toxicology research and the model of isolated liver, we studied the metabolism of methyl isofenphine in the liver. Materials and Methods 1. Animals: Fourteen pure Wistar male rats (230-250 g) were randomly divided into two groups (n = 7 in each group). The control group consisted of ex vivo liver perfusion group, experimental group After 30 minutes of ex vivo perfusion, methyl isoflurane (21.52 mg / kg body weight) was perfused into the medium. 2. Ex vivo hepatic perfusion: The perfusion system was made by Zhang Zi-fa method. The perfusion medium was Krebs-Rin-ger bicarbonate buffer containing 0.15% grapevine, pH7.0 ~ 7.2. Surgical operation to be