本文根据实验结果并参考前人的工作,指出络合性洗脱液增大了过渡金属离子IEC的色谱行为的差别,使得这些离子的HPIEC分离成为可能。定量考察了离子势(Z/r)、失屏参数(R_ε)、络合效应系数(αM(L))、络合剂浓度(C_t)及酸度对保留体积(V_R)或分配系数D的影响,得到了五个经验式: 并通过实验验证了这些式子,指出了它们对实际工作的参考意义. In this paper, based on the experimental results and reference to previous work, it is pointed out that the complexing eluent increases the difference in chromatographic behavior of transition metal ions IEC, making it possible to separate HPIECs of these ions. The effect of ion potential (Z / r), loss of screen parameter (R_ε), complexation coefficient (αM (L)), complexing agent concentration (C_t) and acidity on retention volume (V_R) , We get five empiric formulas: we validate these formulas by experiments and point out their reference significance for practical work.