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应用RP－HPLC方法检测血清中氟脲嘧啶（Fluorouracil）浓度，本方法采用C18分析柱及预柱，流动相为甲醇-0．5％乙酸水溶液（5：95v／v），检测波长266nm，血清样品用乙酸乙酯萃取，氮气吹干，血药浓度在25ng～40μg／ml范围内呈线性关系，相关系数0．9999，最低检测浓度为25ng／ml。生物样品的方法回收率为101．2％～107．7％，日内日间变异系数在1．13％～6．24％（n＝5）。
The concentration of Fluorouracil in serum was determined by RP-HPLC method. The method was based on C18 column and precolumn. The mobile phase consisted of methanol-0.5% acetic acid aqueous solution (5:95 v / v) The sample was extracted with ethyl acetate and dried under nitrogen. The linear range was 25 ng ~ 40 μg / ml with a correlation coefficient of 0.9999 and the lowest detection concentration was 25 ng / ml. The recoveries of biological samples ranged from 101.2% to 107.7%, and the intra-day and intra-day CVs ranged from 1.13% to 6.24% (n = 5).