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Two new triterpenoids, 3β, 6β, 24-trihydroxyolean-12-en-27-oic acid and 3β-acetoxy-6β-hydroxyurs-12-en-27-oic acid, as well as three known triterpenoids were isolated from the rhizomes of Astilbe chinensis. Their structures were elucidated by spectroscopic methods.These compounds showed Anti-cancer activities.