论文部分内容阅读
用平板玻璃纸覆盖技术获得中间球海胆Strongylocentroyusintermedius“红斑病”致病弧菌的胞外产物 (ECP), 并分析了其主要成分。结果表明, 胞外产物中淀粉酶、酪蛋白酶、明胶酶、卵磷脂酶具有活性, 并经感染试验证实, 该胞外产物对海胆有与菌体相同的致病性; 温度可影响胞外产物的蛋白质总含量和酶活性; 胞外产物的剂量对海胆感染率影响显著 (P<0 05 ), 而作用时间对感染率的影响不显著。
The extracellular product (ECP) of Vibrio spp. With Strongylocentroyus intermedius “Osteoporosis” was obtained by the cellophane coverage technique and its main components were analyzed. The results showed that the extracellular products of amylase, casein, gelatinase, lecithinase activity, and confirmed by the infection test, the extracellular product of sea urchins and bacteria have the same pathogenicity; temperature can affect the extracellular products The total protein content and the activity of the enzyme. The dose of the extracellular product had a significant effect on the infection rate of sea urchin (P <0.05), while the effect of the action time on the infection rate was insignificant.