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目的：探讨新一代肿瘤抑制基因-间隙连接基因Cx43及其蛋白产物在人子宫颈癌细胞系HeLa中的表达及意义。方法：应用核酸分子原位杂交和流式细胞仪分析技术，研究人子宫颈癌细胞系HeLa及阳性对照细胞人正常肝细胞系QZG中，间隙连接基因Cx43mRNA及其蛋白产物的表达规律。结果：原位杂交显示，Cx43mRNA在HeLa细胞中呈阴性，在QZG细胞中呈强阳性。流式细胞仪分析示Cx43蛋白在HeLa、QZG细胞中阳性细胞计数率分别为1．9％和99．0％，差异有高度显著性（P＜0．01）。结论：CX43基因及其蛋白产物在HeLa中表达缺失可能与子宫颈癌发生恶性表型相关。
Objective: To investigate the expression and significance of a new generation of tumor suppressor gene, gap junction gene Cx43, and its protein product in human cervical carcinoma HeLa cell line. METHODS: The expression of gap junction gene Cx43 mRNA and its protein products in the normal human hepatocyte cell line QZG of human cervical carcinoma cell line HeLa and positive control cells was studied by using in situ hybridization and flow cytometry analysis. RESULTS: In situ hybridization showed that Cx43 mRNA was negative in HeLa cells and strongly positive in QZG cells. Flow cytometry analysis showed that the positive cell counts of Cx43 protein in HeLa and QZG cells were 1.9% and 99.0%, respectively, and the difference was highly significant (P<0.01). Conclusion: The loss of CX43 gene and its protein product in HeLa may be related to the malignant phenotype of cervical cancer.