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构建以ＣＭＶ启动子控制鼠ＩＬ－３基因表达的复制缺陷型腺病毒（ＡｄＣＭＶＩＬ－３）。将含全部编码序列的鼠ＩＬ－３ｃＤＮＡ克隆于腺病毒穿梭质粒ｐＭＨ４的ＣＭＶ启动子下游，再与含腺病毒基因组的质粒ｐＢＨＧ１０共转染２９３细胞，经细胞内同源重组后产生７个病毒空斑，取其中４个空斑扩增后经双引物ＰＣＲ检测，结果均含有ＩＬ－３及腺病毒特有片段，表明成功地构建了携带鼠ＩＬ－３基因的复制缺陷型重组腺病毒。
A replication-deficient adenovirus (AdCMVIL-3) that controls the expression of murine IL-3 gene with the CMV promoter was constructed. The murine IL-3 cDNA containing the entire coding sequence was cloned downstream of the CMV promoter of the adenoviral shuttle plasmid pMH4 and cotransfected into 293 cells with the adenovirus-containing plasmid pBHG10 to generate 7 viruses empty after intracellular homologous recombination Four plaques were amplified and amplified by double-primer PCR. The results showed that both IL-3 and adenovirus specific fragments were amplified, indicating that replication-defective recombinant adenovirus carrying murine IL-3 gene was successfully constructed.