苁蓉总苷胶囊HPLC指纹图谱研究

来源 :中国中药杂志 | 被引量 : 0次 | 上传用户:lessy123456
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该文建立苁蓉总苷胶囊的HPLC指纹图谱,为其质量评价提供依据。以毛蕊花糖苷为参照物,采用高效液相色谱法,对苁蓉总苷胶囊进行了指纹图谱分析。色谱条件:Waters C18色谱柱(4.6 mm×150 mm,5μm);流动相甲醇-0.1%甲酸溶液梯度洗脱;流速1.0 m L·min-1;检测波长330 nm;柱温30℃。该方法精密度高、重复性好,所测样品中的13个成分均达到基线分离,并且15批成品指纹图谱相似度在0.95以上。该方法准确、可行,是一种简便、有效的中药质量评价手段。 This article established HPLC fingerprint of Cistanche glycosides Capsules to provide the basis for its quality evaluation. The Verbascin glycosides as reference, using high performance liquid chromatography, Cistanche glycosides capsule fingerprint analysis. Chromatographic conditions: Waters C18 column (4.6 mm × 150 mm, 5 μm); mobile phase methanol-0.1% formic acid gradient elution; flow rate 1.0 m L · min-1; detection wavelength 330 nm; The method has high precision and good repeatability. The 13 components in the tested samples all achieved the baseline separation, and the fingerprints of 15 batches of products have the similarity above 0.95. The method is accurate and feasible, which is a simple and effective method to evaluate the quality of Chinese medicine.
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