近年来,Crocker等采用改良的银染核仁组织区(简称AgNORs)方法在常规石蜡组织切片上显示出核仁组织区(NORs)。该技术在国内尚未见报道,本文在参考了国外有关文献的基础上,对石蜡组织切片AgNORs染色方法进行了探索并获得了较好的染色效果,现报告如下。 1.染色液的配制 先配贮存液。A液:将2g明胶用蒸馏水加热溶化至50ml,然后加入2％甲酸水溶液50ml,混匀后盖封并置4℃冰 In recent years, Crocker and his colleagues used modified silver-stained nucleolar organizer regions (AgNORs) to display nucleolar organizer regions (NORs) on conventional paraffin sections. This technique has not been reported in our country. Based on the reference of foreign literature, this paper explores the staining method of the paraffin section of AgNORs and obtains the better dyeing effect. The report is as follows. 1. Dyeing solution preparation first stock solution. Solution A: Dissolve 2 g of gelatin in distilled water to 50 ml, then add 50 ml of 2% formic acid aqueous solution, mix, cover and seal and place at 4 ° C ice