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结核杆菌主要蛋白抗原(Major Protein Antigens of M.tuberculosis,MPTs)是结核杆菌培养上清经过柱、电泳、层析等一系列分离纯化所得的一组蛋白,包括MPT32、45、51、59、64等十几种蛋白质。我们观察了MPT蛋白对小鼠淋巴结细胞增殖反应的影响,探讨MPT蛋白在结核杆菌免疫应答中的作用。 经加热杀死的结核杆菌H37RV(ATCC 27294)标准株(日本国立卫生研究所)用佛氏佐剂配成乳剂,尾根部皮下注射BALB/c和C57BL/6小鼠(日本东大医研所)200μg/只。免疫7天后,无菌取大腿根部淋巴结,研碎过滤,制成细胞悬液,与MTP或PPD(大阪市立大学医学院)共同孵育,测淋巴细胞的增殖反应。实验按常规~3H-TdR掺入法进行,以液闪仪测cpm数。
Mycobacterium tuberculosis major protein antigen (Major Protein Antigens of M.tuberculosis, MPTs) is a group of mycobacterium tuberculosis culture supernatant through a column, electrophoresis, chromatography and a series of isolated and purified a group of proteins, including MPT32,45,51,59,64 More than a dozen kinds of protein. We observed the effect of MPT protein on lymphocyte proliferation in mice and explored the role of MPT protein in the immune response of M. tuberculosis. Mycobacterium tuberculosis H37RV (ATCC 27294) standard strain (Japan National Institutes of Health) which was killed by heating was dosed with Freund’s adjuvant and BALB / c and C57BL / 6 mice were injected subcutaneously at the base of the tail ) 200 μg / bird. Seven days after the immunization, the root thigh lymph nodes were aseptically removed, and the cells were ground and filtrated to prepare cell suspensions. The cells were incubated with MTP or PPD (Osaka City University Medical College) to measure the proliferative response of lymphocytes. The experiment was carried out according to the routine ~ 3H-TdR incorporation method, and the number of cpm was measured by a liquid scintillation counting apparatus.